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KMID : 0362319960210010242
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Journal of Korean Academy of Operative Dentistry
1996 Volume.21 No. 1 p.242 ~ p.253
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Difference in dye penetration into dentinal tubules in vivo and in vitro
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Abstract
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Penetration degree of several dyes into dentinal tubules in vivo was compared with in vitro, and experimental sensitivity of those dyes was investigated in 64 canines of 16 cats. Dentin 1mm below cusp tip was exposed by cross-sectioning with
diamond
disc. Pulp of 32 canines were extirpated through cervical cavities In the presence of smear layer or after acid-etching with 37% phosphoric acid to the exposed dentin surface, acrylic collar and resin cap was attached to keep dyes. 52
mmol/ℓEvans'
blue, 2% Methylene blue, 10% Silver nitrate and 5% Fluorescene were then applicated on each 4 canines respectively for 30 minutes. After rinsing, the canines were ground-sectioned longitudinally and linear dye penetration was measured under
microscope.
@ES The results obtained were as follows;
@1. Evans' blue and Methylene blue penetrated significantly(P<0.05) more in vitro than in vivo only in the cases that exposed dentin surfaces were not etched with acid.
2. Silver nitrate penetrated significantly(P<0.05) more in vitro than in vivo in both cases that exposed dentin surfaes remained intact and were etched with acid.
3. The penetration degree of Fluorescene did not show statistical significance between in vivo and in vitro or in acid-etched and in not acid-etched cases.
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KEYWORD
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